Author(s):
Rajeh A, Alammouri Y, Alzehouri J
Email(s):
abeer.rajeh@damascusuniversity.edu.sy
DOI:
10.52711/0975-4385.2026.00033 
Address:
Rajeh A1, Alammouri Y2*, Alzehouri J3
1PhD Student, Department of Food and an Analytical Chemistry, Faculty of Pharmacy, Damascus University.
2Assistant Professor, International University for Science and Technology, Faculty of Pharmacy.
3Professor, Department of Food and an Analytical Chemistry, Faculty of Pharmacy, Damascus University.
*Corresponding Author
Published In:
Volume - 18,
Issue - 3,
Year - 2026
ABSTRACT:
Fenugreek (Trigonella foenum-graecum L.) is widely recognized for its nutritional and medicinal significance and is commonly used in traditional medicine as well as in functional food formulations. The whole plant, seeds, and sprouted seeds are known to contain a diverse range of bioactive compounds. The present study aimed to evaluate the biochemical composition of fenugreek callus tissue produced through in vitro plant tissue culture techniques. Callus tissue was induced from leaf explants of 15-day-old seedlings cultured on Murashige and Skoog (MS) medium supplemented with naphthalene acetic acid (NAA), either alone or in combination with benzylaminopurine (BAP). Biochemical analyses were performed to determine mineral content, carbohydrate profile, and fatty acid composition. Mineral analysis revealed potassium as the predominant element in the callus tissue, along with detectable levels of sodium, calcium, iron, and zinc. Carbohydrate profiling using high-performance liquid chromatography (HPLC) confirmed the presence of soluble sugars, with fructose and raffinose identified as the dominant components. Lipid analysis conducted by gas chromatography–mass spectrometry (GC–MS) showed that the stored fats were mainly composed of unsaturated fatty acids, particularly a-linolenic acid, linoleic acid, and oleic acid. These findings support the suitability of MS medium supplemented with NAA, with or without BAP, for the cultivation of metabolically active fenugreek callus tissue. Overall, the results indicate that T. foenum-graecum callus tissue is biochemically diverse and represents a promising in vitro source of nutritionally and pharmaceutically valuable compounds.
Cite this article:
Rajeh A, Alammouri Y, Alzehouri J. Biochemical Characterization of Trigonella foenum-graecum Callus Tissue Cultivated in vitro. Research Journal of Pharmacognosy and Phytochemistry. 2026; 18(3):233-7. doi: 10.52711/0975-4385.2026.00033
Cite(Electronic):
Rajeh A, Alammouri Y, Alzehouri J. Biochemical Characterization of Trigonella foenum-graecum Callus Tissue Cultivated in vitro. Research Journal of Pharmacognosy and Phytochemistry. 2026; 18(3):233-7. doi: 10.52711/0975-4385.2026.00033 Available on: https://www.rjpponline.org/AbstractView.aspx?PID=2026-18-3-3
REFERENCES:
1. Petropoulos G.A. Fenugreek: The Genus Trigonella. Taylor and Francis, London, UK. 2002.
2. Flammang A.M., Cifone M.A., Erexson G.L., Stankowski L.F. Jr. Genotoxicity testing of a fenugreek extract. Food and Chemical Toxicology. 2004; 42(11): 1769–1775.
3. Bakhtiar Z., Hassandokht M., Naghavi M., Rezadoost H., Mirjalili M. Fatty acid and nutrient profiles, diosgenin and trigonelline contents, mineral composition, and antioxidant activity of seeds of some Iranian Trigonella L. species. BMC Plant Biology. 2024; 24:669.
4. Bin Jardan Y.A., Ahad A., Raish M., Alam M.A., Al-Mohizea A.M., Al-Jenoobi F.I. Effects of garden cress, fenugreek, and black seed on the pharmacodynamics of metoprolol: an herb–drug interaction study in rats with hypertension. Pharmaceutical Biology. 2021; 59: 1088–1097.
5. Mandal S., Debmandal M. Fenugreek (Trigonella foenum-graecum L.) oils. In: Essential Oils in Food Preservation, Flavor and Safety. Academic Press. 2016; 421–429.
6. Umavandhana R., and Jayanthi, S. Analysis of phytochemical compounds and DPPH radical scavenging activity of Dictyota dichotoma and Halimeda macroloba. Research Journal of Pharmacy and Technology. 2018; 11(8).
7. Gonçalves S., Romano A. Production of plant secondary metabolites using biotechnological tools. In: Secondary Metabolites: Sources and Applications. IntechOpen; 2018: 81–99.
8. Ozyigit I., Dogan I., Ozyigit A., Yalcin B., Erdogan A., Yalcin I., Cabi E., Kaya Y. Production of secondary metabolites using tissue culture-based biotechnological applications. Frontiers in Plant Science. 2023; 14: 1–28.
9. Priyanka Majumdar R. S., and Cynthia M. N. Quick and efficient method for callus culture from stem disc tissue of garlic (Allium sativum L.). Research Journal of Pharmacy and Technology. 2018; 11(6).
10. Pandiyan R., and Ilango K. GC‑MS analysis of bioactive compounds present in different extracts of an endemic tree Huberantha senjiana (Annonaceae) leaves. Research Journal of Pharmacy and Technology. 2023; 16(1).
11. Natarajan P., Singh S., and Balamurugan, K. Gas Chromatography‑Mass Spectrometry (GC‑MS) analysis of bioactive compounds presents in Oeophylla smaragdina. Research Journal of Pharmacy and Technology. 2019; 12(5).
12. Jamshidi S., Lahouti M., Ganjeali A. Assessment of callus growth and bioproduction of diosgenin in callus culture of Trigonella foenum-graecum L. Pharmacology and Life Sciences. 2014; 3: 191–198.
13. Masara D.J., Neamah S.I. Effect of chemical media components and silver nanoparticles on callus induction and accumulation of secondary compounds in Trigonella foenum-graecum L. Iraqi Journal of Agricultural Sciences. 2024; 55(Special Issue): 111–121.
14. Asilbek Kyzy, A., Khasanova, S. R., Kudashkina, N. V., Razzakov, A. K., Boronova, Z. S., Asrankulova, G. A., Razzakova, N. A., Ganyev, R. Z., and Kashfullina, K. I. Phytochemical analysis and biological activity of raw materials of Oxytropis rosea Bunge (Fabaceae) from the flora of Kyrgyzstan. Research Journal of Pharmacy and Technology. 2025; 18(1).
15. AOAC. Official Methods of Analysis. Dairy Products: Cheese. Association of Official Analytical Chemists, Washington DC. 1984; 281–312.
16. AOAC. Official Methods of Analysis. 13th ed. Association of Official Analytical Chemists, Washington DC. 2005.