Author(s): Mahamane Haidara, Adama Denou, Sekou Doumbia, Mamadou L. Diarra, Rokia Sanogo

Email(s): mahamanehaidara83@gmail.com

DOI: 10.52711/0975-4385.2026.00005   

Address: Mahamane Haidara1,2*, Adama Denou1, Sékou Doumbia1, Mamadou L. Diarra1, Rokia Sanogo1,2
1Faculté de Pharmacie de l’Université des Sciences, des Techniques et des Technologies de Bamako (USTTB), Mali
2Institut National de Recherche sur la Médecine et la Pharmacopée Traditionnelles (INRMPT), Mali
*Corresponding Author

Published In:   Volume - 18,      Issue - 1,     Year - 2026


ABSTRACT:
Plants of the genus Tephrosia are very widespread in West Africa. They are used in traditional medicine to treat various diseases mainly inflammatory diseases. The objective of this study was to determine the pharmacognostic parameters of the leafy stems of Tephrosia bracteolata Guill. & Perr., Tephrosia mossiensis A. Chev. and Tephrosia linearis (Willd.) harvested in Mali in order to contribute to the writing of their monographs. The pharmacognostic parameters of the leafy stems of three species of Tephrosia harvested in Mali, have been characterized. Macroscopic, organoleptic features and microscopic elements determined. Extracts were prepared. Main chemical components and antiradical constituents were characterized. The stems of all three Tephrosia species are generally erect, cylindrical, and solid. The leaves are odd-pinnate and alternate with a variable number of pairs of leaflets which are of various shapes. The powders of the leafy stems of the three Tephrosia species had a lichen-green color, an uncharacteristic odor, a tasteless flavor, and a semi-coarse texture. Micrography revealed the presence of specific elements which are multicellular covering trichomes, straight and slightly curved unicellular covering trichomes, and starch grains. The moisture contents were less than 10%. The contents of insoluble ashes in diluted HCl were less than 1%. Main chemical components revealed in all three samples are polyphenols, sterols, and triterpenes. Both aqueous and hydroethanolic extracts exhibited antiradical activity by reducing the DPPH radical. Data from this study contributed to monographs of the leafy stems of these plants and will be helpful in the quality control of leafy stem samples from these plant species.


Cite this article:
Mahamane Haidara, Adama Denou, Sekou Doumbia, Mamadou L. Diarra, Rokia Sanogo. Pharmacognostic Study of three species of Tephrosia Pers. (Fabaceae) harvested in Mali. Research Journal of Pharmacognosy and Phytochemistry. 2026; 18(1):26-0 doi: 10.52711/0975-4385.2026.00005

Cite(Electronic):
Mahamane Haidara, Adama Denou, Sekou Doumbia, Mamadou L. Diarra, Rokia Sanogo. Pharmacognostic Study of three species of Tephrosia Pers. (Fabaceae) harvested in Mali. Research Journal of Pharmacognosy and Phytochemistry. 2026; 18(1):26-0 doi: 10.52711/0975-4385.2026.00005   Available on: https://www.rjpponline.org/AbstractView.aspx?PID=2026-18-1-5


REFERENCES:
1.    Al-Ghamdi FA. Morphological diversity of some Tephrosia species (Fabaceae) in Saudi Arabia. Am J Plant Sci. 2013; 4(3): 543‑8.
2.    Stevenson PC, Kite GC, Lewis GP, Forest F, Nyirenda SP, Belmain SR, Sileshi GW, Veitch NC. Distinct chemotypes of Tephrosia vogelii and implications for their use in pest control and soil enrichment. Phytochemistry. 2012; 78: 135‑46.
3.    Kabongoa RM, Iheanachoa HE, Mageeb AR, Van der Banka M, Boatwrightd JS. Systematics and diversification of the genus Tephrosia and allies (Millettieae, Fabaceae). South Afr J Bot. 2017; 109: 341.
4.    Zhang P, Qin D, Chen J, Zhang Z. Plants in the genus Tephrosia: valuable resources for botanical insecticides. Insects. 2020; 11(10): 721.
5.    Touqeer S, Saeed MA, Ajaib M. A review on the phytochemistry and pharmacology of genus Tephrosia. Phytopharmacology. 2013; 4(3): 598‑637.
6.    Assoumane MI. Utilisations de Tephrosia purpurea (l.) Pers. (Leguminosae) en médecine traditionnelle au Niger [Thèse d’exercice de Pharmacie]. [Bamako, Mali]: Université Kankou Moussa; 2023.
7.    Samuel VJ, Mahesh AR, Murugan V. Phytochemical and pharmacological aspects of Tephrosia genus: A brief review. J Appl Pharm Sci. 2019; 9(3): 117‑25.
8.    Fofie YBN, Coulibaly K, Traore K, Odoh E, Zihiri GN, Kone-Bamba D. Pharmacognostic study of Ziziphus mauritiana LAM.(Rhamnaceae). Int J Pharmacogn. 2018; 5(6): 354‑9.
9.    Yvette FNB, Kiyinlma C, Diénéba KB. Phamacognostic study of Ocimum gratissimum Linn.: Pharmafood plant. J Pharmacogn Phytochem. 2014;2(5):74‑9.
10.    European Directorate for the Quality of Medicines &, HealthCare of the Council of Europe. European Pharmacopoeia. Tenth Edition. Vol. I. Strasbourg Cedex, France: Council of Europe; 2019.
11.    World Health Organization. Quality control methods for herbal materials. Updated edition of Quality control methods for medicinal plant materials, 1998. Geneva: World Health Organization; 2011. 173 p.
12.    Ravikumar VR, Shastikaa SS, Loganayagi M, Maheswari V, Natarajavel R, Karishma T. Pharmacognostical Studies on Traditional Drug of" Nalvelai" Cleome gynandra (Capparaceae). Res J Pharmacogn Phytochem. 2025; 17(1): 5‑8.
13.    Bruneton J. Pharmacognosie, Phytochimie, Plantes médicinales. 5e édition. Lavoisier, Paris: Technique et Documentation, Lavoisier Paris, France; 2016. 1487 p.
14.    Thorat B, Pangrikar P. Phytochemical Analysis of Fruits and Leave of Psidium guajava L. Res J Pharmacogn Phytochem. 2025; 17(2): 107‑10.
15.    Blois M. Antioxidant determinations by the use of stable free radicals. Nature. 1958; 181(4617).
16.    Haidara M, Dénou A, Arama DP, Sanogo R. Activité antiradicalaire des extraits des feuilles de Ampelocissus grantii (Baker) Planch.(Vitaceae), une plante utilisée traditionnellement contre les maladies hémorroïdaires au Mali. Pharmacopée Médecine Tradit Afr. 2023; 22(1): 67‑71.
17.    Marston A. Thin-layer chromatography with biological detection in phytochemistry. J Chromatogr A. 2011; 1218(19): 2676‑83.
18.    Chanda S. Importance of pharmacognostic study of medicinal plants: An overview. J Pharmacogn Phytochem. 2014;2(5):69‑73.
19.    Rajabudeen E, Saravana G, Subramanian M. Pharmacognostical Studies on Tephrosia villosa (L.) Pers. (Fabaceae). Res J Pharmacogn Phytochem. 29 déc 2014; 6(4): 190‑4.
20.    Egharevba GO, Dosumu OO, Oguntoye SO, Njinga NS, Dahunsi SO, Hamid AA, Anand A, Amtul Z, Priyanka U. Antidiabetic, antioxidant and antimicrobial activities of extracts of Tephrosia bracteolata leaves. Heliyon. août 2019; 5(8): e02275.
21.    Garba MH, Ampitan TO, AbdulAzeez RA, Tawakaltu AA, Fajobi EA, Jeje CA. In vivo Trypanostatic Activity of Tephrosia linearis Extract on Trypanosoma evansi. Fountain J Nat Appl Sci. 2021; 10(2): 15‑24.
22.    Stagos D. Antioxidant activity of polyphenolic plant extracts. Vol. 9, Antioxidants. MDPI; 2019.

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